How to load basis metabolite to do Quest quantification

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  • #1422
    Xiaoyan Xu
    Participant

    I used NMRScope-B to simulate several metabolites signal and saved as mrui format to be used in Quest quantification. But when I tried to load the file (choose a metabolite–>Add one), I got an error message “you can not use this Mrui file”. Does anybody know what I did wrong? or give me some help on how to do Quest quantification using basis function?
    Thank you.

    Xiaoyan Xu
    Columbia University

    #1426
    Jon Dudley
    Participant

    It might be that your spectrum and basis file have different sizes (may be the case if you weren’t careful to adjust the values in NMRscopeB to match your acquisition parameters). Try quantifying the mrui file of your simulated signals with HSVD and then loading the results files for QUEST quantification.

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